Journal: Nature Communications

Article Title: Binary mineral nanoparticles enable intravascular delivery of metal ions to tumors for metalloimmunotherapy

doi: 10.1038/s41467-025-68279-y

Figure Lengend Snippet: a Scheme of tumor challenge and treatment schedule for 4T1-luc2 orthotopic model. b Average tumor volumes and ( c ) individual tumor growth curves in tumor-bearing mice across various treatment groups. d Bioluminescence images of 4T1-luc2 tumor-bearing mice at 3 weeks after the last treatment. e Representative IHC images showing expression of TGF-β1, epithelial markers (Epcam, E-Cadherin), and mesenchymal markers (N-Cadherin, Vimentin) in collected tumors across different treatment groups (Scale bar: 120 μm). f Statistical comparisons of the protein expression levels quantified in ( e ) . g , h Evaluation of lung metastasis in 4T1-luc2 tumor models. g Representative fluorescent images showing luc2 protein expression in lung tissues (Scale bar: 120 μm). h Statistical comparisons of the luc2 + cell density quantified in ( g ). i–n Evaluation of survival and long-term immune memory in the 4T1-luc2 orthotopic model. i Treatment scheme for the study. j Bioluminescence images tracking spontaneous metastasis over the study period. k Survival curves of 4T1-luc2 tumored mice. l–n Tumor rechallenge study (day 180) to assess immune memory. Naïve mice were used as the control group. ( l ) Individual tumor growth curves, ( m ) average tumor volumes, and ( n ) survival rate. e , g (1) Control (PBS), (2) BMNs, (3) aPD1, (4) BMNs + aPD1. b , f , h , m Data are presented as mean ± SD with ( b ) n = 5 mice, ( f , h ) n = 5,5,5,3 mice, and ( m ) n = 6,4 mice. b , m Two-way ANOVA with Tukey’s multiple comparison tests. f , h One-way ANOVA with Tukey’s multiple comparison tests. k , n Survival curves were obtained using the Kaplan–Meier method and compared by the log-rank Mantel-Cox test. Source data are provided as a Source Data file.

Article Snippet: On day 6, mice were divided into 4 groups ( n = 6), and they received IV injections of PBS or BMNs (Na + : 1.5 mg/kg, Ca 2+ : 60 mg/kg) through on days 6, 9, and 12 and anti-mouse PD-1 antibody (aPD1) (BE0033-2, Bio X Cell) (10 mg/kg) through IP injections on days 7, 10, and 13.

Techniques: Expressing, Control, Comparison

Journal: Molecular Therapy

Article Title: DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure

doi: 10.1016/j.ymthe.2020.02.007

Figure Lengend Snippet: Characteristics of Anti-CTLA-4 and Anti-PD-1 mAb-Encoding pDNA Constructs

Article Snippet: Additionally, Absolute Antibody murinized the chimeric IgG1 anti-PD-1 mAb sequences, i.e., mutations were introduced in the variable regions to improve similarity to mouse germline sequences [p(CMV- m aPD-1-HC 1 ), p(CMV- m aPD-1-LC)].

Techniques: Plasmid Preparation

Journal: Molecular Therapy

Article Title: DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure

doi: 10.1016/j.ymthe.2020.02.007

Figure Lengend Snippet: Engineering of a DNA-Based Anti-CTLA-4 mAb and DNA-Based Anti-PD-1 mAb

Article Snippet: Additionally, Absolute Antibody murinized the chimeric IgG1 anti-PD-1 mAb sequences, i.e., mutations were introduced in the variable regions to improve similarity to mouse germline sequences [p(CMV- m aPD-1-HC 1 ), p(CMV- m aPD-1-LC)].

Techniques: